MetaboAnalyst

Batch Correction

MetaboAnalyst currently supports nine well-established methods (ComBat, EigenMS, QC-RLSC, ANCOVA, RUV-random, RUV2, RUVseq, NOMIS and CCMN) for batch effect correction.

Batches can be uploaded as multiple individual files or a single merged file. In the former case, only overlapped features across all batches will be used. In the later case, batch information should be provided in the table. More details are provided below for each scenario;
The automated (default) will return the results with least distance among batches.
EigenMS is not supported for large files (> 5M), please use MetaboAnalystR in this case.
QC-RLSC requires injection order (only available for 'Upload A Single Merged File')

Upload multiple batch files

Please upload your data set (one at a time):

Data format:

Correction method:

Evaluation target:

Missing value estimation:

Data label:

Data file:

Choose

An example with 3 batches can be downloaded here. Please unzip first and then upload each individually.

Upload a single merged file

Please upload your data table:

Or try our test data :

Examples

Description

Data 1

Data correction based on generic structure of data.

Data 2

Data correction with QC samples as reference.

Data 3

Data correction with QC or internal standards as reference.

Data 4

Data correction with multiple references.